Journal: AIDS Research and Human Retroviruses

Article Title: The Majority of HIV Type 1 DNA in Circulating CD4 + T Lymphocytes Is Present in Non-Gut-Homing Resting Memory CD4 + T Cells

doi: 10.1089/aid.2012.0351

Figure Lengend Snippet: Subject Demographics and CD4 + T Cell Subsets as Percentage of CD3 + CD4 + T Cells

Article Snippet: After enrichment with Dynabeads Untouched Human CD4 T Cell kit (Invitrogen, Oslo, Norway), 1–2×10 8 enriched CD4 + T cells were stained using the following three panels of monoclonal antibody cocktails into 14 subsets: CD3-PerCP-Cy5.5, CD4 + -PE-Cy7 (BD Biosciences, San Jose, CA), and CD45RO-ECD (Beckman Coulter, Hialeah, FL) were used in all three panels to define CD4 + T cells and to mark memory phenotype.

Techniques: Infection, Cell Counting

Journal: AIDS Research and Human Retroviruses

Article Title: The Majority of HIV Type 1 DNA in Circulating CD4 + T Lymphocytes Is Present in Non-Gut-Homing Resting Memory CD4 + T Cells

doi: 10.1089/aid.2012.0351

Figure Lengend Snippet: Gut-homing memory CD4+ T cell population in antiretroviral treatment (ART)-suppressed primary HIV-1 infection (PHI) and chronic HIV-1 infection (CHI) patients. Memory gut-homing CD4+ T cells were identified by expression of CD45RO and integrin β7. (A) The first panel shows the clearly defined β1+ and β7+ populations from the CD3+CD4+CD45RO+α4+(CD49d) cells within peripheral blood mononuclear cells (PBMCs). The second panel depicts sorting based on CD3+CD4+CD45RO+β7+ cells. These cells are clearly associated with α4, with 98% of the β7+ cells shown to be positive for α4. The presence of β7+ cells within CD45RO+CD4+ T cells is shown for one representative subject studied in the third panel. The proportions of memory gut-homing and memory non-gut-homing CD3+CD4+ T cell subsets are shown with the majority of cells demonstrated to be non-gut-homing β7−CD4+ T cells. (B) Scatter plots of medians and interquartile range (IQR) of absolute total and integrated HIV-1 DNA copies/500 ng DNA are shown for CD3+CD4+, CD3+CD4+CD45RO−, CD3+CD4+CD45RO+β7+, and CD3+CD4+CD45RO+β7− T cell subsets from CHI patients. Each individual patient is represented by a unique symbol. Both the gut-homing and non-gut-homing memory CD4+ T cell subsets contained a significantly greater number of total HIV-1 DNA copies/500 ng DNA than the naive CD4+ T cell subsets (p=0.016 for both comparisons). (C) Scatter plots of the median percentage and IQR of total and integrated HIV-1 DNA in either integrin β7+CD45RO+CD4+ T cells (16% and 19.5%, respectively) or β7−CD45RO+CD4+ T cells (84% and 80.5%, respectively) are shown for CHI patients with each individual patient represented by a unique symbol. (D) Corresponding scatter plots of medians and IQR of absolute total HIV-1 DNA copies/500 ng DNA are shown for ART-suppressed and PHI patients.

Article Snippet: After enrichment with Dynabeads Untouched Human CD4 T Cell kit (Invitrogen, Oslo, Norway), 1–2×10 8 enriched CD4 + T cells were stained using the following three panels of monoclonal antibody cocktails into 14 subsets: CD3-PerCP-Cy5.5, CD4 + -PE-Cy7 (BD Biosciences, San Jose, CA), and CD45RO-ECD (Beckman Coulter, Hialeah, FL) were used in all three panels to define CD4 + T cells and to mark memory phenotype.

Techniques: Infection, Expressing

Journal: AIDS Research and Human Retroviruses

Article Title: The Majority of HIV Type 1 DNA in Circulating CD4 + T Lymphocytes Is Present in Non-Gut-Homing Resting Memory CD4 + T Cells

doi: 10.1089/aid.2012.0351

Figure Lengend Snippet: Memory T regulatory and memory CD127high CD4+ T cell subsets in CHI. Memory Treg CD3+CD4+ cells were identified within the CD45RO+ population by high expression of CD25 and dim expression of CD127. (A) Representative histograms for one CHI subject are shown. The percentages for each population are shown. (B) Scatter plots of medians and IQR of total and integrated HIV-1 DNA copies/500 ng DNA are shown for CD3+CD4+, CD3+CD4+CD45RO−, CD3+CD4+CD45RO+CD25+CD127dim, and CD3+CD4+CD45RO+CD127high T cell subsets from CHI patients. Each individual patient is represented by a unique symbol. Total HIV-1 DNA copies/500 ng of DNA significantly resided within the Treg and CD127high memory CD4+ T cell subsets compared to the naive CD4+ T cell subset (p=0.03 for both comparisons). (C) Scatter plots of median percentage and IQR of total and integrated HIV-1 DNA in either Treg CD25+CD127dim (5% and 7%, respectively) or CD127high (95% and 93%, respectively) subsets of memory CD4+ T cells are shown for CHI patients. Each individual patient is represented by a unique symbol.

Article Snippet: After enrichment with Dynabeads Untouched Human CD4 T Cell kit (Invitrogen, Oslo, Norway), 1–2×10 8 enriched CD4 + T cells were stained using the following three panels of monoclonal antibody cocktails into 14 subsets: CD3-PerCP-Cy5.5, CD4 + -PE-Cy7 (BD Biosciences, San Jose, CA), and CD45RO-ECD (Beckman Coulter, Hialeah, FL) were used in all three panels to define CD4 + T cells and to mark memory phenotype.

Techniques: Expressing

Journal: AIDS Research and Human Retroviruses

Article Title: The Majority of HIV Type 1 DNA in Circulating CD4 + T Lymphocytes Is Present in Non-Gut-Homing Resting Memory CD4 + T Cells

doi: 10.1089/aid.2012.0351

Figure Lengend Snippet: Activated memory cells identified by their expression of CD38. (A) Representative histograms for a PHI subject and a CHI subject gated on activated memory CD4+ T cells are shown. Percentages for each population are also shown. (B) Scatter plots of medians and IQR of total and integrated HIV-1 DNA copies/500 ng DNA are shown for CD3+CD4+CD45RO−, CD3+CD4+CD45RO+CD38−, and CD3+CD4+CD45RO+CD38+ T cell subsets from CHI patients. Each individual patient is represented by a unique symbol. (C) Scatter plots of median percentage and IQR of total and integrated HIV-1 DNA in CD38+ (92% and 72%, respectively) or CD38− (8% and 28%, respectively) subsets of memory CD45RO+CD4+ T cells are shown for CHI patients. Each individual patient is represented by a unique symbol.

Article Snippet: After enrichment with Dynabeads Untouched Human CD4 T Cell kit (Invitrogen, Oslo, Norway), 1–2×10 8 enriched CD4 + T cells were stained using the following three panels of monoclonal antibody cocktails into 14 subsets: CD3-PerCP-Cy5.5, CD4 + -PE-Cy7 (BD Biosciences, San Jose, CA), and CD45RO-ECD (Beckman Coulter, Hialeah, FL) were used in all three panels to define CD4 + T cells and to mark memory phenotype.

Techniques: Expressing